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Home
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Technical Reference Library
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The Molecular Probes Handbook
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Ultrasensitive Detection Technology—Chapter 6
Ultrasensitive Detection Technology—Chapter 6
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Chapter 6—Ultrasensitive Detection Technology
Introduction to Signal Amplification—Section 6.1
TSA and Other Peroxidase-Based Signal Amplification Techniques—Section 6.2
Phosphatase-Based Signal Amplification Techniques—Section 6.3
Phycobiliproteins—Section 6.4
Microspheres—Section 6.5
Qdot Nanocrystals—Section 6.6
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Signal Amplification—Section 6.1
Signal Amplification: Why and How
Primary and Secondary Detection Reagents
TSA and Other Peroxidase-Based Signal Amplification Techniques—Section 6.2
Principles of Tyramide Signal Amplification
A Variety of Kits for TSA Detection
Applying TSA Technology to Cells and Tissues
Chromogenic and Chemiluminescent Peroxidase Substrates
Peroxidase-Based Amplex ELISA Kits
Phosphatase-Based Signal Amplification Techniques—Section 6.3
Principles of ELF Signal Amplification
Applications of ELF Signal Amplification
Chromogenic Phosphatase Substrate
CSPD and CDP-
Star
Chemiluminescent ELISA Detection
Phycobiliproteins—Section 6.4
Spectral Characteristics of Phycobiliproteins
Pure Phycobiliproteins
Phycobiliprotein Conjugates
Zenon Antibody Labeling Technology
Microspheres—Section 6.5
Properties of Molecular Probes Fluorescent and Nonfluorescent Microspheres
Applications for Fluorescent Microspheres
FluoSpheres Fluorescent Microspheres
Fluorescent Microsphere Starter Kits
Fluorescent Microspheres for Educational Purposes
TransFluoSpheres Fluorescent Microspheres: Tools for Multicolor Detection
BlockAid Blocking Solution
Qdot Nanocrystals—Section 6.6
Properties of Qdot Nanocrystals
Qdot Nanocrystal Products and Applications
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Chapter 6—Ultrasensitive Detection Technology
(2.6 MB)
Related Tables
Tyramide Signal Amplification (TSA) Kits—Table 6.1
Spectral data for B-PE, R-PE and APC—Table 6.2
Tandem conjugates of R-phycoerythrin (R-PE)—Table 6.3
Tandem conjugates of allophycocyanin (APC)—Table 6.4
Molecular Probes yellow-green–fluorescent FluoSpheres beads compared with other commercially available yellow-green–fluorescent microspheres—Table 6.5
Fluorescein equivalents in Molecular Probes yellow-green–fluorescent FluoSpheres beads—Table 6.6
Summary of FluoSpheres fluorescent microspheres—Table 6.7
Summary of biotin-, streptavidin- and NeutrAvidin biotin-binding protein–labeled FluoSpheres microspheres—Table 6.8
Summary of TransFluoSpheres fluorescent microspheres—Table 6.9
Extinction coefficients of Qdot nanocrystal streptavidin conjugates at ultraviolet and visible wavelengths—Table 6.10
Qdot nanocrystal secondary antibody conjugates—Table 6.11
Qdot nanocrystal anti–human CD antibody conjugates—Table 6.12