Various volume capacities of Zeba Spin Desalting Columns

Thermo Scientific Zeba Spin Desalting Columns are made of low protein binding polypropylene and compatible with a wide range of standard laboratory instruments and consumables. 

Our Zeba columns are designed to be compatible with most swinging-bucket or fixed-angle bench- or floor-model centrifuges (please verify head clearance before use), and are available in five volume capacities and two molecular weight cut-offs (MWCOs), 7K and 40K.

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Choose the right Zeba Spin Desalting Column for you

 Micro spin columnZeba 0.5 mL spin columnZeba 2 mL spin columnZeba 5 mL spin columnZeba 10 mL spin columnStacks of 96-well clear microplates
FormatMicro Spin Column0.5 mL Spin Column2 mL Spin Column5 mL Spin Column10 mL Spin Column96-well Filter Plates
Resin bed
volume		75 μL0.5 mL2 mL5 mL10 mLN/A
Volume capacity
(7K MWCO)		2–12 μL30–130 μL200–700 μL500–2,000 μL700–4,000 μL20–100 µL
Volume capacity
(40K MWCO)		5–14 μL70–200 μL200–900 μL300–2,000 μL1,000–4,000 μL20–100 µL
7K MWCO pack sizes
Cat. No.		89877, 8987889882, 8988389889, 8989089891, 8989289893, 8989489807, 89808
40K MWCO pack sizes
Cat. No.		A57756, A57758A57759, A57760A57761, A57762 A57763, A57764A57765, A57766A57767, A57768

Compare Zeba spin desalting columns

Table 1. Comparison of recommended sample volume capacity of common spin desalting products.

Table comparing sample volume capacity of common spin desalting products

Protein recovery comparison using Zeba Protein Desalting Columns with 7K and 40K MWCO resin

Redeveloped Zeba 40K Spin Desalting Columns demonstrate improved protein recovery for a variety of proteins in the 40 kDa and above molecular weight range.

 Recovery*
ProteinMolecular Size7K Zeba Column40K Zeba Column
Bacitracin1.5 kDa15%12%
Aprotinin6.5 kDa58%37%
Ubiquitin8.5 kDa78%51%
Lactalbumin14 kDa88%64%
Trypsin Inhibitor21 kDa94%76%
Carbonic Anhydrase30 kDa90%79%
Ovalbumin42 kDa95%95%
BSA67 kDa94%93%
Rabbit IgG150 kDa92%92%

*Recovery of 100 uL sample volume, with a loaded concentration of 0.5 mg/mL

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