Green Fluorescent Protein (GFP) excitation shown as dotted line and emission shown as solid blue histogram
4488FITC488510(in buffer) 2
(in antifade) 2		microscopy, flow cytometry

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. It is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.

We offer a series of GFP Invitrogen CellLight fusion constructs of signal peptides or cell structure proteins with emGFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments.

To complement these tools, we also offer anti-GFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. Our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and CellLight fusion proteins.

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Experimental data using Green Fluorescent Protein (GFP)
Overlay of fluorescence and differential interference contrast (DIC) micrographs showing GFP-transfected NIH 3T3 cells (yellow), DAPI-stained nuclei (light-blue) and untransfected cells with DAPI-stained nuclei (bright-blue)

Dual labeling of NIH 3T3 cells with GFP and anti-GFP antibody. NIH 3T3 cells were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, plated and allowed to attach and proliferate. The cells were fixed and labeled with our GFP Polyclonal Antibody, Alexa Fluor 594. Cells expressing GFP showed dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI stains the nuclei with a light-blue fluorescence. In the untransfected cells, the DAPI-stained nuclei exhibited a bright-blue fluorescence. 

Live-cell imaging of HeLa cells showing mitochondria-GFP (green), DNA-Hoechst (blue) and talin-RFP (red) using CellLight reagents

Live-cell imaging of HeLa cells with CellLight reagents using BacMam technology. HeLa cells were transduced with CellLight Mitochondria-GFP, BacMam 2.0 and CellLight Talin-RFP. The following day, cells were stained with Hoechst 33342 and live-cell imaging was performed using DeltaVision Core microscope and standard DAPI/FITC/TRITC filter sets.