Ulysis™ Alexa Fluor™ 546 Nucleic Acid Labeling Kit
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Invitrogen™

Ulysis™ Alexa Fluor™ 546 Nucleic Acid Labeling Kit

ULYSIS™ Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagentRead more
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Catalog number U21652
Price (USD)
612.00
Each
Add to cart
Price (USD)
612.00
Each
Add to cart
ULYSIS™ Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast—just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

ULYSIS™ Nucleic Acid Labeling Specifications:
• Dye (Ex/Em): Alexa Fluor™ 546 (555/570 nm)
• Labeling reaction is complete in as little as 15 minutes
• Available in several Alexa Fluor™ dye colors

The Resulting Labeled Probe is useful for:
• Dot, northern, and Southern blots
• RNA and DNA in situ hybridization
• Multicolor fluorescence in situ hybridization (mFISH)
• Comparative genome hybridization (CGH)
• Microarray analysis

Reliable Labeling With the Universal Linkage System
We developed this series of ULYSIS™ kits to enable rapid and simple coupling of our Alexa Fluor™ dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS™), is based on the use of a platinum dye complex (owned by KREATECH Diagnostics) that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is Fast and Easy
The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS™ complex can be accomplished through the use of a simple spin-column procedure. DNA longer than ∼1,000 base pairs requires a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling, review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook or view a list of our kits.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
FormatKit
Label or DyeAlexa Fluor 546
Final Product TypeProbes (Labeled RNA), Probes (Labeled DNA), Oligos (Labeled)
Labeling TargetDNA (General), Oligos, RNA (General)
Includes Label or DyeYes
Labeling MethodDirect Labeling
Product LineAlexa Fluor™, Ulysis™
Product TypeNucleic Acid Labeling Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

View all Ulysis™ Alexa Fluor™ 546 Nucleic Acid Labeling Kit FAQs