Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)
Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)
Invitrogen™

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker GreenRead more
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Catalog number T34078
Price (USD)
184.00
Each
Add to cart
Price (USD)
184.00
Each
Add to cart
Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation Wavelength Range494/522
Dye TypeOregon Green™ 488
Quantity60 slides
Product LineTubulinTracker™
Shipping ConditionApproved for shipment on Wet or Dry Ice
Label TypeUV-Vis Labels
Product TypeTubulin Tracker
SubCellular LocalizationTubulin
Unit SizeEach
Contents & Storage
Contains sufficient material for at least 60 slides at 1 mL / slide and 1 x working concentration.

1 x 20 μg Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate)

1 x 1 mL Pluronic F-127, 20% solution in DMSO

Frequently asked questions (FAQs)

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.