SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
Invitrogen™

SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, makingRead more
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Catalog number S7020
Price (USD)
336.00
Each
Add to cart
Price (USD)
336.00
Each
Add to cart
SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.

Also available as a room-temperature-stable, ready-to-use solution: NucGreen Dead 488 Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

•  Impermeant to live cells
•  Excitation/Emission: 504/523 nm
•  Use with 488 Argon-ion laser
•  Works with mammalian cells and both Gram-positive and Gram-negative bacteria

Simple Viability Determination
SYTOX Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX Green will result in bright green fluorescence with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450–490 nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.

Part of the Invitrogen Family of Cell Viability Stains and Assays
SYTOX stains for dead cells are available in a variety of colors, including red, blue, and orange. Additionally, Invitrogen has incorporated SYTOX stains into a number of assays for apoptosis, cell viability, and metabolism.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Find out more about all SYTOX Products.



Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Excitation Wavelength Range504 nm
Dye TypeCell-Permeant
FormatTube(s)
For Use With (Equipment)Flow Cytometer
Quantity250 μL
Volume (Metric)250 μL
Detection MethodFluorescence
Emission523 nm
FormSolution
Product LineSYTOX
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids, Nucleus
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to mount my Qdot secondary-labeled tissue samples in HistoMount mounting medium, but my Qdot 705 conjugate overlaps with your Qnuclear Deep Red nuclear label. What other nuclear label would you recommend, that is compatible with HistoMount mounting medium?

We recommend using SYTOX Green stain, which you can image using a FITC filter and we have shown to be compatible with HistoMount mounting medium. Some have used DAPI, but there have been some issues with slight quenching and spectral shifting of DAPI into green or even red wavelengths.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.