SYPRO™ Protein Gel Stains
SYPRO™ Protein Gel Stains
SYPRO™ Protein Gel Stains
SYPRO™ Protein Gel Stains
Invitrogen™

SYPRO™ Protein Gel Stains

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE).
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Catalog NumberDescriptionExcitation/Emission
S6651SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 10 x 50 μL300, 470/570 nm
S6650SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 500 μL300, 470/570 nm
S12010SYPRO™ Tangerine Protein Gel Stain (5000X in DMSO), 500 μL300, 490/640 nm
S6653SYPRO™ Red Protein Gel Stain (5000X in DMSO), 500 μL300, 550/630 nm
S6654
also known as S-6654
SYPRO™ Red Protein Gel Stain (5000X in DMSO), 10 x 50 μL300, 550/630 nm
S12001SYPRO™ Ruby Protein Gel Stain (1X), 200 mL280, 450/610 nm
S12000SYPRO™ Ruby Protein Gel Stain (1X), 1 L280, 450/610 nm
S21900SYPRO™ Ruby Protein Gel Stain (1X), 5 L280, 450/610 nm
S12000X3SYPRO™ Ruby Protein Gel Stain (1X), 3 x 1 L280, 450/610 nm
Catalog number S6651
Price (USD)
371.00
Each
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Description:
SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 10 x 50 μL
Excitation/Emission:
300, 470/570 nm
Request bulk or custom format
Price (USD)
371.00
Each
Add to cart
SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.
SYPRO Ruby Protein Gel Stain features include:
• Convenient—ready-to-use formulation
• High sensitivity—detection to 0.25 to 1 ng
• Effective staining for 1D and 2D gels
• Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
• Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

SYPRO Orange and SYPRO Red protein gel stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10–60 min
• Best for 1D gels
• SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
• SYPRO Red: Ex: 300, 550 nm, Em: 630 nm

SYPRO Tangerine Protein Gel Stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10 to 60 minutes
• Best for 1D gels
• Does not require fixation with acetic acid
• Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionSYPRO™ Orange Protein Gel Stain (5000X in DMSO), 10 x 50 μL
Detection LocationIn-Gel Detection
Concentration5000X in DMSO
Detection MethodFluorescence
Label or DyeSYPRO Orange
Product TypeProtein Gel Stain
Product LineSYPRO™
Quantity10 x 50 μL
Shipping ConditionRoom Temperature
Target MoleculeProtein
Excitation/Emission300, 470/570 nm
Unit SizeEach
Contents & Storage
Supplied as a 5000X concentrate in DMSO. Store at room temperature and protect from light.

Frequently asked questions (FAQs)

If I increase the final concentration of my SYPRO Orange or SYPRO Red staining solution above 1X, can I increase the signal of my stained proteins?

No. Dye concentrations higher than 1X do not give better detection. Instead, background fluorescence increases and, as the dye concentration increases, the dye becomes self-quenching and the signal actually decreases.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I dry my SYPRO Ruby, SYPRO Orange, or SYPRO Red stained gels?

Gels stained with SYPRO dyes can be dried between sheets of cellophane, although there is sometimes a slight decrease in sensitivity. If the gels are dried onto paper, the light will scatter and the sensitivity will decrease. Other plastics are not recommended, as the plastic typically used is not transparent to UV light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I pre-stain proteins with SYPRO Ruby, SYPRO Orange or SYPRO Red Protein Gel Stains and then run them through a gel?

No. Loading solutions contain so much SDS that SYPRO Ruby, SYPRO Orange and SYPRO Red dyes simply localize in the free SDS and bind very little of the proteins. Proteins can be covalently pre-labeled with ATTO-TAG CBQCA (Cat. No. A2333), DDAO succinimidyl ester (Cat. No. C34553) or TAMRA-succinimidyl ester (Cat. No. C2211) dyes, or the TC-FLAsH Expression Analysis Detection Kits (Cat. No. A10067 for orange fluorescence, Cat. No. A10068 for red fluorescence) prior to electrophoresis without affecting protein migration through the gel.

SYPRO Orange or SYPRO Red Protein Gel Stain can be diluted 5000-fold into the cathode (upper) buffer tank to stain proteins during electrophoresis without affecting migration. The problem with doing this is that there is considerable background fluorescence in the gels from the dye interacting with SDS. This can be reduced after electrophoresis by destaining in 7.5% acetic acid for 15-60 minutes. This method also results in poorer protein sensitivity than the standard post-staining method, requires the same amount of time before the gel can be imaged, and contaminates the electrophoresis apparatus.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.