SlowFade™ Diamond Antifade Mountant
SlowFade™ Diamond Antifade Mountant
Invitrogen™

SlowFade™ Diamond Antifade Mountant

SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscopeRead more
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Catalog NumberVolume (Metric)
S369672 mL
S369635 x 2 mL
S3697210 mL
Catalog number S36967
Price (USD)
76.75
Each
Add to cart
Volume (Metric):
2 mL
Price (USD)
76.75
Each
Add to cart
SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. You can use SlowFade Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background.

Features of SlowFade Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that does not cure; samples can be viewed immediately
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Maintains fluorescence signal—little to no quenching

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 μm, try SlowFade Glass Antifade Mountant. It is a RIMS mountant with refractive index of 1.52.

Select the best SlowFade Antifade reagents for your experimental needs ›

SlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive index similar to glass or immersion oil, then ProLong Glass Antifade Mountant is recommended. With the no-curing-required properties of SlowFade Diamond mountant, samples can be evaluated and imaged right after labeling.

Get photobleaching protection across the entire visible and IR spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, SlowFade Diamond mountant helps provide the best protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. SlowFade Diamond mountant does not quench, and its ability to provide brighter signal, against no background, helps ensure that you obtain the best-quality, high resolution images for publishing.

Stable signals during imaging and for immediate viewing
SlowFade Diamond mountant is the ideal option for analyzing samples right after labeling. Its antifade properties for both fluorescent dyes and fluorescent proteins preserves signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. A slide sealant can be used to preserve the slides for days or weeks. If longer archiving is desired, then the SlowFade Diamond mountant can be washed away with PBS, and the samples remounted with ProLong Diamond Antifade Mountant.

Achieve excellent refractive index for crisp images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. SlowFade™ Diamond mountant has a refractive index of 1.42 and is recommended for cultured cells or thin slices of tissue (less then 10 μm in thickness).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeAntifade Mountant
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Product LineSlowFade™
Reagent TypeMounting Solution, Antifade Solution
Volume (Metric)2 mL
Unit SizeEach
Contents & Storage
Contains 1 dropper bottle contaning 2 mL reagent.

• Store at 2-8°C or frozen at -5 to -30°C.
• Protect from light.
• Stable for at least 6 months after receiving.

Frequently asked questions (FAQs)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to mount my dye-labeled cells in an antifade mounting medium to keep the dyes from photobleaching. Which mounting medium do you recommend?

As dyes are illuminated for imaging, they will fade, or “photobleach”, leading to unwanted dimming and lower detection efficiency over time. An antifade mounting medium can greatly reduce photobleaching. If you wish to label live cells, use of ProLong Live Antifade Reagent is helpful. If you wish to mount fixed cells after labeling, and then image immediately and then discard, SlowFade Diamond Antifade Mountant stays liquid but has good refractive index. If you wish to mount your sample and then archive the slides, ProLong Diamond Antifade Mountant will harden to a better refractive index and allow for archiving of the sample for up to several weeks, or even months. Unlike other antifade mounting media, these work well with fluorescent proteins for immediate viewing (archiving fluorescent proteins is not possible), and they are packaged with or without DAPI. More information on these can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-gold-antifade.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.