SYBR™ Safe DNA Gel Stain in 0.5X TBE
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SYBR™ Safe DNA Gel Stain in 0.5X TBE
Invitrogen™

SYBR™ Safe DNA Gel Stain in 0.5X TBE

Green features
SYBR Safe DNA gel stain was developed specifically for reduced mutagenicity – to be safer than ethidium bromide for stainingRead more
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Catalog NumberQuantity
S331014 L
S331001 L
Catalog number S33101
Price (USD)
452.00
Each
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Quantity:
4 L
Request bulk or custom format
Price (USD)
452.00
Each
Add to cart
SYBR Safe DNA gel stain was developed specifically for reduced mutagenicity – to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe stain is not only less mutagenic than ethidium bromide, but SYBR Safe stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ∼280 and ∼502 nm, and an emission maximum at ∼530 nm. SYBR Safe DNA gel stain is also available as 1 L (S-33100) of ready-to-use solution. The SYBR Safe DNA Gel Stain Starter Kit (S-33110) includes 1 L of SYBR Safe DNA gel stain and one photographic filter (S-37100).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Label or DyeSYBR Safe
Product TypeDNA Gel Stain
Shelf Life6 months from date of purchase
Green FeaturesLess hazardous
Quantity4 L
Shipping ConditionRoom Temperature
Target MoleculeDNA
Unit SizeEach
Contents & Storage
• Supplied as a ready-to-use solution in 0.5X TBE

Store at room temperature in the original container.

Frequently asked questions (FAQs)

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.