SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

The Alexa Fluor™ 750 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree ofRead more
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Catalog number S30046
Price (USD)
916.00
Each
Add to cart
Price (USD)
916.00
Each
Add to cart
The Alexa Fluor™ 750 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree of labeling for in vivo imaging applications (DOL; ∼2) over a 6-fold antibody concentration range with no adjustments in reaction volume, dye concentration, or antibody concentration necessary. Using this procedure, optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injection into animals.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeLabeling Kit
Quantity1 kit
Detection MethodFluorescence
Excitation/Emission749/775
Labeling Scale0.5 to 3 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™, SAIVI™
Label or DyeAlexa Fluor 750
ColorInfrared
Labeling TargetAntibodies
Label TypeAlexa Fluor Dyes
Unit SizeEach
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.