SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO
SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO
Invitrogen™

SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cellRead more
Have Questions?
Catalog number S11368
Price (USD)
361.00
Each
Add to cart
Price (USD)
361.00
Each
Add to cart
SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide (P-1304).

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Excitation Wavelength Range547 nm
Dye TypeCell-Permeant
FormatTube(s)
For Use With (Equipment)Fluorescence Microscope
Quantity250 μL
Volume (Metric)250 μL
Detection MethodFluorescence
Emission570 nm
FormSolution
Product LineSYTOX
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.