SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO
SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO
Invitrogen™

SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, makingRead more
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Catalog number S11348
Price (USD)
341.00
Each
Add to cart
Price (USD)
341.00
Each
Add to cart
SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. The excitation/emission maxima for the dye bound to DNA are 444/480 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Excitation Wavelength Range444 nm
Dye TypeCell-Permeant
FormatTube(s)
For Use With (Equipment)Fluorescence Microscope
Quantity250 μL
Volume (Metric)250 μL
Detection MethodFluorescence
Emission480 nm
FormSolution
Product LineSYTOX
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I leave SYTOX Blue Nucleic Acid Stain - 5 mM Solution in DMSO on my cells during imaging?

SYTOX dyes are only tested for end-point assays, not for leaving in cell solutions long term. Therefore, we do not recommend using SYTOX Blue Nucleic Acid Stain - 5 mM Solution in DMSO for long-term imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.