FreeStyle™ 293-F Cells
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Gibco™

FreeStyle™ 293-F Cells

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™Read more
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Catalog number R79007
Price (USD)
1,038.00
Each
Add to cart
Price (USD)
1,038.00
Each
Add to cart
The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium.
• Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent.
• Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media.
• Permits transfection of cells at large volumes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineFreeStyle™ 293-F Cells
Cell Type293-F
SpeciesHuman
For Use With (Application)Protein Expression and Transfection
Media AdaptationFreeStyle™ 293 Expression Medium
Product LineFreestyle™
Product Type293-F Cell
Quantity1 mL
System TypeFreeStyle™
Unit SizeEach
Contents & Storage
1 vial of 1 x 107 cells. Store in liquid nitrogen.

Frequently asked questions (FAQs)

I do not have FreeStyle 293-F cells. Can I use a different 293 cell line with the FreeStyle 293 Expression System?

Other 293 cell lines may be used with the FreeStyle 293 Expression System. However, they would have to be adapted to serum-free suspension culture in FreeStyle 293 Expression Medium and evaluated for transfection and expression.

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to grow FreeStyle 293-F cells in 8% CO2 when using the FreeStyle 293 Expression Medium? If 8% CO2 cannot be arranged, can I supplement the media with an additional reagent?

Our recommendation of 8% CO2 for the optimal growth of Freestyle 293 cells in Freestyle 293 Expression Medium is based on the bicarbonate content in the media and growth conditions of the cells observed by R&D. That said, you may use lower concentrations of CO2 if necessary (5% is common) to grow these cells. You may observe slightly slower growth kinetics or lower cell concentrations, but functionally the entire system will still work.

View all FreeStyle™ 293-F Cells FAQs