ReadyProbes 2.5% Normal Chicken Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF)Read more
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Catalog number R37626
Price (USD)
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ReadyProbes 2.5% Normal Chicken Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a chicken secondary antibody is used. Incubating cells/tissue with diluted serum from the same species as the host of the secondary antibody typically reduces non-specific binding. Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Chicken Serum and incubate for 30–60 minutes at room temperature. Ready Probes 2.5% Normal Chicken Serum can also be used to dilute primary and secondary antibodies.
Ready Probes 2.5% Normal Chicken Serum is provided as a ready-to-use reagent with no further dilution or preparation required. The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay. The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
Product TypeChicken Serum
Quantity50 mL
Product LineReadyProbes™
Reagent TypeBlocking Reagent
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit Size50 mL
Contents & Storage
Provided as 50-mL solution at ready-to-use (1X) concentration in a clear plastic dropper bottle.
Store at 2–8°C.
When stored as directed, product can be used for 6 months after receipt.
Frequently asked questions (FAQs)
Can I use ReadyProbes 2.5% Normal Chicken Serum (1X) as an antibody diluent for both primary and secondary antibodies?
Yes, ReadyProbes 2.5% Normal Chicken Serum (1X) can be used as an antibody diluent for both primary and secondary antibodies, except for HRP-conjugated antibodies. The sodium azide added as a preservative is an inhibitor of horseradish peroxidase.
When would I use goat, horse, or chicken serum for blocking versus FBS or BSA?
For blocking, you should use a serum of the same or closely related species of the secondary antibody being used. If using a secondary antibody that was raised in goat or sheep, use goat serum (Cat. No. R37624 or 31873) for blocking; if using a secondary antibody raised in donkey or horse, use horse serum (Cat. No. R37625 or 31874) for blocking; if using a secondary antibody raised in chicken, use chicken serum (Cat. No. R37626) for blocking. FBS and BSA are alternatives when a specific species of serum is not available.