TO-PRO™3 Ready Flow™ Reagent
TO-PRO™3 Ready Flow™ Reagent
Invitrogen™

TO-PRO™3 Ready Flow™ Reagent

TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells inRead more
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Catalog number R37170
Price (USD)
377.00
Each
Add to cart
Price (USD)
377.00
Each
Add to cart
TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid staining of dead cells without wash steps

TO-PRO 3 Ready Flow Reagent is a ready-to-use, direct-to-sample dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops form the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

TO-PRO 3 Ready Flow Reagent is membrane impermeant and therefore excluded from viable cells. It binds selectively to double-stranded DNA. This dye has excitation/emission maxima of 642/661 nm.

Specifications
Cell PermeabilityImpermeant
ColorFar-Red
DescriptionTO-PRO™3 Ready Flow™ Reagent
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product TypeReagent
Dye TypeCell-Impermeant
Emission661 nm
Excitation Wavelength Range642 nm
FormSolution
FormatBottle(s)
Product LineReady Flow™, TO-PRO™3
Quantity120 assays
SolubilityDMSO (Dimethylsulfoxide)
Sub Cellular LocalizationNucleus
Unit SizeEach
Contents & Storage
Includes 2 dropper bottles, store at room temperature.

Frequently asked questions (FAQs)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.