Hoechst 33342 Ready Flow™ Reagent
Hoechst 33342 Ready Flow™ Reagent
Invitrogen™

Hoechst 33342 Ready Flow™ Reagent

Hoechst 33342 Ready Flow™ Reagent is a bright, easy-to-use cell-permeant stain for cell cycle analysis on a flow cytometer. ThisRead more
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Catalog number R37165
Price (USD)
252.00
Each
Add to cart
Price (USD)
252.00
Each
Add to cart
Hoechst 33342 Ready Flow™ Reagent is a bright, easy-to-use cell-permeant stain for cell cycle analysis on a flow cytometer. This dye emits blue fluorescence when bound to double-stranded DNA with an emission maximum at 461 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Cell-permeable and effective in fixed and live-cell staining for cell cycle

Hoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. This dye binds into the minor grooves of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. Hoechst 33342 Ready Flow Reagent is excited by UV light and has peak emission at 460 nm.

Specifications
ColorBlue
Excitation Wavelength Range361/497
Dye TypeCell-Permeant
Quantity120 assays
Product LineReady Flow™
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleus
Unit SizeEach
Contents & Storage
2 dropper bottles, store at room temperature

Frequently asked questions (FAQs)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.