ViewRNA™ ISH Cell Assay Kit
ViewRNA™ ISH Cell Assay Kit
Invitrogen™

ViewRNA™ ISH Cell Assay Kit

The ViewRNA™ ISH Cell Assay is a direct fluorescence RNA in situ hybridization method that enables the simultaneous detection ofRead more
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Catalog number QVC0001
Price (USD)
1,968.00
Each
Add to cart
Price (USD)
1,968.00
Each
Add to cart
The ViewRNA™ ISH Cell Assay is a direct fluorescence RNA in situ hybridization method that enables the simultaneous detection of one to four RNA targets (either mRNA or non-coding RNA) at single copy sensitivity and single cell resolution using fluorescence microscopes or high-content imagers. Unlike traditional FISH techniques which are generally limited by high background and low sensitivity, the assay uses proprietary chemistry for the target specific probe sets (RNA FISH probes) and branch DNA signal amplification (bDNA) for detection of specific signal. The fluorescence RNA in situ hybridization assay has four main steps: sample preparation, target hybridization, signal amplification, and detection. This ViewRNA ISH assay kit can detect up to three RNA but can be combined with the ViewRNA™ 740 Module to add the fourth probe.

Required additional components:
10X PBS (cat. no. QVC0508)
ViewRNA™ - Detergent Solution QC (cat. No. QVC0509)
ViewRNA™ - Wash Buffer Set (cat. no. QG0507)
ViewRNA™ ISH Cell Accessory Kit (cat. no. QVC0700) intended to provide many of the required components, not supplied in the reagent kit, in order to perform the assay.
ViewRNA™ Probe Sets are not included in the assay and are designed for use with the ViewRNA ISH Cell Assays. Visit our website to view a complete listing of over 6,500 synthesized Probe Sets. By request, new Probe Sets can be designed and synthesized in less than two weeks with no additional costs.

To add the 4th RNA probe:
ViewRNA™ ISH Cell 740 Module (cat. no. QVC0200) designed to be used in conjunction with ViewRNA™ ISH Cell Assay and allows analysis of an additional RNA target in the 740 channel (AlexaFluor 750) See the package insert for a complete list of materials provided in the kit.

Reported Applications
Microscopy

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 kit
TypeDetection Assay Kit
Unit SizeEach
Contents & Storage
Components sufficient for 1-plex to 3-plex (AlexaFluor 488, 546 and 647) assay in 24- well plate format (24 samples), 96 assays when using cover slips mounted on glass slides

Frequently asked questions (FAQs)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The ViewRNA ISH Cell Assay Kit user manual lists an accessory kit, Cat. No. QVC0700 which has been discontinued. Do you offer or suggest alternatives for the items in the accessory kit that have also been discontinued?

Please contact our Technical Support team at cellanalysis.support@thermofisher.com for the list of suggested alternatives.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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