QuantiGene™ Sample Processing Kit, cultured cells
QuantiGene™ Sample Processing Kit, cultured cells
Invitrogen™

QuantiGene™ Sample Processing Kit, cultured cells

This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for useRead more
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Catalog NumberQuantity
QS010350 Plates
QS01002 Plates
QS010110 Plates
QS01025 x 10 Plates
Catalog number QS0103
Price (USD)
1,298.00
Each
Add to cart
Quantity:
50 Plates
Price (USD)
1,298.00
Each
Add to cart

This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA targets and QuantiGene Plex DNA assays for DNA targets.

  • Easy to follow instructions
  • Preserves the integrity of the RNA/DNA without degradation
  • Choose the method that matches your cultured cell sample

What you get

The QuantiGene Sample Processing Kit for cultured cells contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing cell lysates from cultured cells.

Success in cell lysis

Key factors that influence the complete lysis of the cells include:

  • The lysis mixture
  • Proteinase K
  • Vortexing or pipetting of the sample
  • Storage of the lysates at -80°C
  • Incubation at 50°C

The protocols provided with the QuantiGene Sample Processing Kit for cultured cells use a combination of these factors to ensure complete lysis of the samples. In addition, the lysis mixture provides the proper ionic conditions for the overnight hybridization in the QuantiGene assays.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Sample TypeCell Cultures
Product LineQuantiGene™
Product TypeSample Processing Kit
Quantity50 Plates
Shipping ConditionBlue Ice
Unit SizeEach
Contents & Storage
• 350 mL Lysis Mixture, store at 15°C to 30°C
• 3.5 mL Proteinase K (50 μg/μL), store at -20°C

Frequently asked questions (FAQs)

Can samples in TRIzol solution be run in the QuantiGene Plex assay?

No, samples in TRIzol solution cannot be run in the QuantiGene Plex assay. However, RNA purified from these samples can be run in the QuantiGene Plex assay.

Can I use the QuantiGene Plex assay with bacteria?

Although the kits were not originally validated for bacteria, we have guidelines for preparing bacterial samples for QuantiGene Plex assays: http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf

Do you offer QuantiGene Plex assay custom services where I can send my samples for you to process?

Yes, please reach out to your Technical Sales Specialist for more information or fill out this form: https://www.thermofisher.com/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html

Can I detect genomic DNA using the QuantiGene Plex assay?

Under the hybridization conditions that are used for the QuantiGene Plex assay, genomic DNA remains double-stranded. Therefore, it is not possible for the probes to bind to DNA. The signal from the probe sets comes only from RNA.

Can I use other lysis buffers with QuantiGene Plex assay samples?

We only recommend the use of QuantiGene Lysis Mixture for preparing cell and blood lysates. The Lysis Mixture has been optimized to provide stability of RNA in the lysates. In addition, QuantiGene Lysis Mixture provides the appropriate buffer conditions for the overnight hybridization.

View all QuantiGene™ Sample Processing Kit, cultured cells FAQs