Yeast Nitrogen Base
Yeast Nitrogen Base
Invitrogen™

Yeast Nitrogen Base

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammoniumRead more
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Catalog number Q30009
Price (USD)
900.00
Each
Add to cart
Price (USD)
900.00
Each
Add to cart
Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammonium sulfate but does not contain amino acids. Both bulk and convenient single-use pouches are available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormPowder
Media TypeYNB
Preparation MethodAutoclave
Product TypeMicrobiology Media
Target Organism ClassP. pastoris, S. cerevisiae
Final Product TypeLiquid Medium
MaterialPowder Reagents
Packaging Type500 g/Bottle
Quantity500 g
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Each bottle contains 500 g of reagent. Use 67 g to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media.

Store at room temperature. Guaranteed stable for 12 months when properly stored.

Frequently asked questions (FAQs)

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.