Qtracker™ 655 Vascular Labels
Qtracker™ 655 Vascular Labels
Invitrogen™

Qtracker™ 655 Vascular Labels

Qtracker™ non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascularRead more
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Catalog number Q21021MP
Price (USD)
774.00
Each
Add to cart
Price (USD)
774.00
Each
Add to cart
Qtracker™ non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascular structure using small animal in vivo imaging (SAIVI) techniques. These nanocrystals exhibit intense fluorescence with red-shifted emission for increased tissue penetration, and have a PEG surface coating specially developed to minimize nonspecific interactions and reduce immune response by the tissue. Because the PEG surface coating does not contain reactive functional groups, the Qtracker™ non-targeted quantum dots are retained in circulation longer and can be imaged for up to 3 hours with a single injection or for longer periods of time with additional injections.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

• Qtracker™ 655 label has Ex/Em (405-615/655) nm
• Designed for small animal in vivo imaging
• Introduced via tail vein injection, can be imaged for up to 3 hours after injection
• Available in four colors—565 nm, 655 nm, 705 nm, or 800 nm emission

Read more about SAIVI and about applications for Qdot™ nanocrystals.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2 μM
Dye TypeQdot Nanocrystals
Quantity200 μL
Product LineQTRACKER™, Qdot™
Reagent TypeVascular Imaging Reagents
Shipping ConditionRoom Temperature
TechniqueIn Vivo Imaging
Product TypeVascular Label
Unit SizeEach
Contents & Storage
Contains 200 μl Qtracker™ non-targeted quantum dots (2 μM solution in 50 mM borate buffer, pH 8.3). Store at 2–6°C. Do not freeze. Stable for at least 6 months.

Frequently asked questions (FAQs)

When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?

Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What amount of Qtracker non-targeted reagent should I inject to image vasculature?

A recommended starting dosage is 25-50 µL of Qtracker reagent diluted to the desired injection volume with PBS or normal saline. Qtracker reagent should be diluted immediately prior to injection. DO NOT STORE DILUTED. You will need to determine the optimal dosage for your experimental models.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Qtracker™ 655 Vascular Labels FAQs