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PolarScreen™ PPARγ-Competitor Assay Kit, Green
The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminalRead more
Catalog number PV6136
Price (USD)
2,070.00
Each
Price (USD)
2,070.00
Each
The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
For Use With (Equipment)Microplate Reader
Quantity800 x 20 μL
Packaging384-well Plate
Assay EntryBiochemical competitive binding
Excitation/Emission485/535
Gene ID (Entrez)5468
ReadoutEnd Point
Shipping ConditionDry Ice
Target EntryPPARG, PPAR gamma, NR1C3
Product LinePolarScreen™
For Use With (Application)Competitive Binding Assay, Fluorescence Polarization
LigandPPAR
Product TypeAssay Kit
Unit SizeEach
Contents & Storage
PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)