Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Invitrogen™

Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from mini-size gels using the PowerRead more
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Catalog NumberQuantity
PB7220
Catalog number PB7220
Price (USD)
240.00
Each
Add to cart
Price (USD)
240.00
Each
Add to cart
These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from mini-size gels using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of nitrocellulose membranes:
High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity
High-binding affinity—provides excellent protein binding (209 μg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting
Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from standard precast or homemade mini gels (8 cm x 8 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The nitrocellulose membrane (0.45 μM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). These pre-cut membrane/filter paper sandwiches fit mini sized gels.

Learn more about the Power Blotter System ›

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Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size
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Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Length (Metric)7 cm
Width (Metric)8.4 cm
FormatTransfer Stack
MaterialNitrocellulose
Pore Size0.45 μm
Shipping ConditionRoom Temperature
Dimensions (LxW)Mini, 7 x 8.4 cm
Thickness0.85 mm
Product LinePower Blotter
Sufficient For20 Blots
Unit SizeEach
Contents & Storage
20 mini-size transfer stacks (8 cm x 8 cm) composed of 20 sheets of nitrocellulose and 80 sheets of filter paper (0.85 mm thickness)

Frequently asked questions (FAQs)

What is the difference between the Power Blotter System and the Power Blotter XL System?

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to do gel staining with the Power Blotter System/Power Blotter XL System?

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the material used for the anode and cathode plates of the Power Blotter Cassette/Power Blotter Cassette XL?

The top (cathode) is stainless steel and the bottom (anode) is platinum-coated stainless steel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The central European or UK power cord for the Power Blotter System/Power Blotter XL System does not work in Switzerland. Do you offer a Swiss power cord?

Yes, you may purchase the Universal Cable Kit (Cat. No. 4377117).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I have lost the power cord for the Power Blotter Station. Is it sold as a stand-alone item?

Here are the SKUs for the power cord. Please order the one appropriate for your region:

- Cat. No. 84858: Power Cord with C13 Connector, North America
- Cat. No. 84859: Power Cord with C13 Connector, Continental Europe and South Korea
- Cat. No. 84860: Power Cord with C13 Connector, United Kingdom and Singapore
- Cat. No. 84855: Chinese 10 Amp Cordset w/ C13 Connector
- Cat. No. 84856: Japanese 15 Amp Cordset w/ C13 Connector
- Cat. No. 84857: Australian 10 Amp Cordset w/ C13 Connector

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.