ProLong™ Diamond Antifade Mountant
ProLong™ Diamond Antifade Mountant
ProLong™ Diamond Antifade Mountant
ProLong™ Diamond Antifade Mountant
Invitrogen™

ProLong™ Diamond Antifade Mountant

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples. ProLong DiamondRead more
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Catalog NumberDescription
P36965ProLong™ Diamond Antifade Mountant, 1 x 2 mL
P36961ProLong™ Diamond Antifade Mountant, 5 x 2 mL
P36970ProLong™ Diamond Antifade Mountant, 1 x 10 mL
Catalog number P36965
Price (USD)
86.25
Each
Add to cart
Description:
ProLong™ Diamond Antifade Mountant, 1 x 2 mL
Price (USD)
86.25
Each
Add to cart

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples. ProLong Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high-definition fluorescent images.

ProLong Diamond Antifade Mountant comes mixed and ready-to-use in a 2 mL dropper bottle or 10 mL bottle. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant does not discolor or shrink when cured, enabling high-quality fluorescent images months after mounting your sample. ProLong Diamond mountant will cure to an RI of 1.47.

Get photobleaching protection across the entire visible and IR spectrums
ProLong Diamond Antifade Mountant’s ability to provide brighter signal, against low background, helps ensure that you get high-resolution images. ProLong Diamond provides superior protection against photobleaching and will not quench your fluorophores. It can be used with an array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5 or fluorescent proteins. For immediate viewing of the sample without curing, SlowFade Diamond Antifade Mountant is optimal.

Archiving fluorescently labeled samples with excellent RI
ProLong Diamond mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins. Please note that ProLong Glass mountants are recommended for samples > 10 microns and all immersion oil imaging applications. It is also ideal for preserving signal strength during prolonged imaging sessions, such as repeated imaging under confocal laser scanning illumination, or with super resolution microscopy. Additionally, the curing property of ProLong Diamond means you do not need to use traditional acrylic resins (i.e., fingernail polish), which can damage microscope objectives if it comes into contact with them. The refractive index determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 Vial
Product TypeAntifade Mountant
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Product LineProLong™
Reagent TypeMounting Solution, Antifade Solution
Volume (Metric)2 mL
DescriptionProLong™ Diamond Antifade Mountant, 1 x 2 mL
Unit SizeEach
Contents & Storage
Contains 1 dropper bottle contaning 2 mL reagent.

• Store at 2-8°C.
• Protect from light.
• Stable for at least 6 months after receiving.

Frequently asked questions (FAQs)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I use ProLong Gold Antifade Mountant to mount my slides, should I seal the edges?

ProLong Gold Antifade Mountant hardens overnight at room temperature. For short-term storage (a couple of weeks) you do not need to seal the edges of the coverslip, and the sample should be stable. Beyond that time, though, some dye conjugates will have an off-rate into the medium, so cold storage is recommended. Sealing the edges will prevent long-term discoloration (golden color) from developing around the edges of the coverslip as the anti-oxidants oxidize.

The edges may be sealed with melted paraffin, VALAP (1:1:1 vaseline, lanolin, paraffin) or epoxy glue. Nail polish is not recommended as various components of nail polish may diffuse into the mountant and quench fluorescent dyes.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I mounted my cells in ProLong antifade mounting medium, but now I want to go back and re-label them. Is there a way I can unmount the coverslip after it has cured (hardened)?

Yes. Put the slide in a Coplin jar or beaker filled with warm (37oC) PBS buffer and let it sit, no agitation is required. The hardened ProLong mountant will swell and may slide off or be easily dislodged. If cells are adherent to the coverslip, make certain the coverslip side containing the cell or tissue sample does not land face down in the container or become scratched upon handling. Remove the coverslip, wash a couple of times, and proceed with re-staining and re-mounting in new ProLong mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using ProLong antifade mounting medium. Do I need to let it cure before imaging? Do I need to seal the edges of the coverslip?

You can image before it cures (hardens), and it will still slow photobleaching, but you have to let it cure overnight to get the best refractive index (resolution). There is no need to seal the edges. In fact, if you seal before it cures, it won't cure correctly. If you are archiving the slide for more than a month, though, seal the edges with resin, paraffin or VALAP (1:1:1 vaseline, lanolin, paraffin) after it cures or there may be slight discoloration along the edges over time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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