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Propidium Iodide - 1.0 mg/mL Solution in Water
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells,Read more
Catalog number P3566
Price (USD)
103.00
Each
Price (USD)
103.00
Each
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.
PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.
Learn more about propidium iodide, and propidium iodide containing products
Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.
PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.
PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.
Learn more about propidium iodide, and propidium iodide containing products
Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypePropidium Iodide
Dye Typepropidium iodide
Emission533⁄617
FormSolution
Quantity10 mL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationCytoplasm & Cytosol
Unit SizeEach
Contents & Storage
Contains 1 bottle of propidium iodide(1.0 mg/mL solution in water).
Store in refrigerator (2–8°C) and protect from light.
Store in refrigerator (2–8°C) and protect from light.