AmpliTaq™ DNA Polymerase, LD (Low DNA)
Inquire about OEM or Commercial Supply version of this product here.
For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
This product will be discontinued on April 30th, 2025. For superior performance upgrade to: Cat no. N8080160.
AmpliTaq™ DNA Polymerase, LD (Low DNA)
Applied Biosystems™

AmpliTaq™ DNA Polymerase, LD (Low DNA)

Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
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Catalog NumberIncludes
N8080157Buffer I
N8080158Buffer II
Catalog number N8080157
Price (USD)
249.00
Each
Add to cart
Includes:
Buffer I
Request bulk or custom format
Price (USD)
249.00
Each
Add to cart
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

This highly purified enzyme preparation ensures that non-target, false-positive PCR products will be effectively minimized when amplifying bacterial sequences. This is especially useful for low-copy-number PCR amplifications. The enzyme is quality-control tested to verify that fewer than 10 copies of bacterial 16S ribosomal RNA gene sequences are present in a standard 2.5 unit aliquot.

AmpliTaq DNA Polymerase, LD, is supplied with either 10X PCR Buffer I or with 10X PCR Buffer II and MgCl2 Solution.

Features

  • Minimizes non-specific PCR products when amplifying bacterial targets
  • Highly purified enzyme making it particularly useful for low copy number amplifications

Notes

  • See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone enzyme
GC-Rich PCR PerformanceHigh
PolymeraseAmpliTaq DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Product TypeDNA Polymerase
Purity or Quality GradeLD (Low DNA)
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
IncludesBuffer I
Unit SizeEach
Contents & Storage
• AmpliTaq DNA Polymerase, LD (5 U/μL), 25 μL
• 10X PCR Buffer I, 1.5 mL

Store at -15°C to -30°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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