Rat Glial Precursor Cells
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Rat Glial Precursor Cells
Gibco™

Rat Glial Precursor Cells

Gibco Rat Glial Precursor Cells are isolated and cryopreserved from the cortex of newborn Sprague Dawley Rats. A flexible, ready-to-use,Read more
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Catalog number N7746100
also known as N7746-100
Price (USD)
778.00
Each
Add to cart
Price (USD)
778.00
Each
Add to cart
Gibco Rat Glial Precursor Cells are isolated and cryopreserved from the cortex of newborn Sprague Dawley Rats. A flexible, ready-to-use, and quality alternative to fresh precursor cells, each vial contains 1 million cells per mL. These cells consistently yield a post-thaw viability of >50%. Upon proliferation in StemPro NSC SFM, >80% of the cells retain their undifferentiated phenotype with the capability to differentiate into oligodendrocytes and astrocytes.

The cells are isolated under sterile conditions, expanded in complete StemPro NSC SFM, and cryopreserved at Passage 2. Each vial can be expanded once to give >2-fold increase in cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AgeNewborn (14 days or less)
Cell TypeGlial Precursor Cells
SpeciesRat
TissueCortex
No. of Cells1 x 106
Tested ForCell Purity, Differentiation Potential, Sterility, Viability, Mycoplasma
Unit SizeEach
Contents & Storage
Store in liquid nitrogen.

Frequently asked questions (FAQs)

How long can I culture Gibco Rat Glial Precursor Cells?

These cells can be thawed and passaged once before using in experiments. The single passage will yield a 2-fold increase of thawed cells. Cells do not expand significantly beyond first passage post-thaw.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where were Gibco Rat Glial Precursor Cells (Cat. No. N7746100) isolated from? How many passages do these cells go through before being cryopreserved?

Rat Glial Precursor Cells were isolated from the cortex of new born Sprague Dawley rats at day 2 after birth, and cryopreserved at passage 2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the growth factors or cytokines used in neural stem cell (NSC) culture?

For NSC expansion, the following growth factors are used: recombinant EGF (Cat. No. PHG0314), recombinant FGF-basic (Cat. No. PHG0024), and recombinant VEGF (Cat. No. PHC9394). In addition, several neurotrophins such as BDNF Cat. No. 10908010), NT-3, CNTF (Cat. No. PHC7015), and GDNF (Cat. No. PHC7044)are also used in the related studies.

Information pertaining to whether a specific product has been tested against the WHO Reference Standard can typically be located on the product page or Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture human neural stem cells (NSCs)?

Human NSCs can grow in Gibco StemPro NSC SFM (Cat. No. A1050901) on dishes pre-coated with Gibco Geltrex Matrix or Gibco CELLstart substrate. Alternatively, if the goal is to obtain neurons, NSCs can also be grown on Neurobasal medium supplemented with Gibco B-27 supplements without vitamin A on a pre-coated dish.

How can I characterize neural stem cells (NSCs)?

NSCs are generally characterized by their ability to form neurospheres when plated at cloning density (Nat Methods 2:333 (2005)). NSCs can also be characterized by (1) RT-PCR of Sox1, Sox2, and Nestin or (2) immunohistochemical staining for nestin, Pax6, Sox2, and Ki67.

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