The TRF spectrum option is useful for spectral analysis, assay and label development, and optimization purpose. For example, when a new TRF chelate label is developed, TRF spectrum measurement can be used to analyze the spectra when a lot of label is loaded in the well.
When the TRF spectrum option is used, the sensitivity is not sufficient for actual TRF assays as the instrument sensitivity decreases by about 200-fold. Hence, the TRF spectrum option should not be used for normal TRF assay applications. For optimal results, the TRF spectrum option should be used for TRF spectral scanning but not for running actual TRF assays. Actual TRF assays must be performed using filter-based TRF optics in the LAT module.
If the Varioskan unit does not have TRF optics in the LAT module, TRF spectral scanning cannot be performed. In this case, TRF spectral scanning can only be performed using TRF-based filters.
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