MitoTracker™ Deep Red FM Dye, for flow cytometry
MitoTracker™ Deep Red FM Dye, for flow cytometry
Invitrogen™

MitoTracker™ Deep Red FM Dye, for flow cytometry

Our MitoTracker dyes for flow cytometry are cell-permeant reagents that label mitochondria in live cells. They are provided at anRead more
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Catalog number M46753
Price (USD)
220.00
5 x 20 tests
Add to cart
Price (USD)
220.00
5 x 20 tests
Add to cart
Our MitoTracker dyes for flow cytometry are cell-permeant reagents that label mitochondria in live cells. They are provided at an optimized concentration with protocols for flow cytometry. This allows for more precise analysis with both conventional and spectral flow cytometry. A range of available colors are detected with standard flow cytometry filters.

Features of our MitoTracker dyes for flow cytometry include:
Bright—allow for easy detection of mitochondria using flow cytometry without the need for special filters
Robust—allow for generation of high quality and reproducible data
Convenient—optimized for flow cytometry; all essential reagents for labeling mitochondria in a single kit

Bright
Our Mitotracker dyes for flow cytometry were designed to provide a bright signal with minimal overlap into adjacent channels, simplifying spectral compensation. Since mitochondria can be discriminated using a single flow cytometer channel, these viability dyes are an ideal choice for multi-color experiments. Additionally, their unique spectral fingerprints are a valuable addition to spectral flow cytometry experiments.

Robust
These Mitotracker dyes are cell-permeant mitochondrion-selective dyes. These dyes are consistent, reliable, and stable, allowing for the generation of high-quality data.

Convenient
These dyes have been conveniently packaged at a concentration that is optimal for flow cytometry. Dissolve a vial of dye in anhydrous DMSO to provide enough dye for 20 assays. Use immediately or store at -20°C, minimizing freeze-thaw cycles.

How it works
To label mitochondria, simply incubate cells in the recommended concentrations of the Mitotracker dyes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

Colors available
Our Mitotracker dyes for flow cytometry are available in a variety of colors to meet your multi-color flow cytometry needs.

Specifications
For Use With (Application)Flow Cytometry
Format5 x 20 tests
Label or DyeDye
No. of Tests100 tests
Quantity5 vials
Regulatory StatusRUO
Shipping ConditionRoom Temperature
Unit Size5 x 20 tests

Frequently asked questions (FAQs)

Our facility does not allow flow cytometry of live cells. Can I fix the cells after staining with MitoTracker Deep Red FM dye?

Yes, this is possible because MitoTracker Deep Red FM dye is retained upon fixation. See Table 2 in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07510.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference in using MitoTracker dyes for flow cytometry compared to imaging?

Because of the greater sensitivity of a flow cytometer, you typically use a much lower concentration of reagent than what is typically used for imaging applications. The MitoTracker dyes, for flow cytometry have been optimized for ease of use for most flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any difference between the newer MitoTracker dyes for flow cytometry products and the original MitoTracker dye products?

The newer MitoTracker dyes “for flow cytometry” and the original MitoTracker dyes differ only in the packaging. The original MitoTracker dye products are shipped as 20 vials with 50 µg per vial. The newer MitoTracker dyes “for flow cytometry” products are shipped as 5 vials with a proprietary amount of dye per vial. The amount of dye per vial is optimized for flow cytometry experiments. Each vial provides sufficient reagent for 20 flow cytometry experiments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.