MitoProbe™ TMRM Assay Kit for Flow Cytometry
MitoProbe™ TMRM Assay Kit for Flow Cytometry
Thermo Scientific™

MitoProbe™ TMRM Assay Kit for Flow Cytometry

The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCPRead more
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Catalog number M20036
Price (USD)
281.00
Each
Add to cart
Price (USD)
281.00
Each
Add to cart
The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP for the induction of mitochondrial membrane depolarization, if desired.

• Study mitochondrial membrane potential in live cells
• Quick, easy protocol

Determining the state of mitochondrial health can be achieved by detecting changes in the mitochondrial membrane potential. In a healthy cell with active mitochondria, TMRM is readily sequestered, thereby emitting a red-orange fluorescent signal. When apoptosis is induced or a treatment like CCCP is applied, the mitochondrial membrane is depolarized and the TMRM signal diminishes.

Specifications
Detection MethodFluorescence
For Use With (Application)TMRM Assay
For Use With (Equipment)Flow Cytometer
Product TypeFlow Cytometry Assay Kit
Emission561
FormatVial(s)
Quantity100 assays
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
  • 1 vial TMRM (100 tests)
  • 1 vial 50 mM CCCP
  • Store at -5°C to -30°C.

Frequently asked questions (FAQs)

In the MitoProbe TMRM Assay Kit for Flow Cytometry, should CCCP be present when adding the TMRM?

Yes. The CCCP should be first added to the cell sample, incubated for 5 mins, and then the TMRM should be added and incubated for another 30 mins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How does the MitoProbe TMRM Assay Kit for Flow Cytometry (Cat. No.M20036) differ from the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is provided as a 100 µM solution of TMRM in DMSO (5 vials, 100 µL in each vial). The MitoProbe TMRM Assay Kit for Flow Cytometry provides the TMRM as a 20 µM stock solution in DMSO (1 vial, 100 µL) and a vial of CCCP. Both kits have the stock solutions as a 1000X concentration, but final working concentrations required for imaging applications are 5-fold higher than what is needed for flow cytometry analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.