SilverQuest™ Silver Staining Kit
SilverQuest™ Silver Staining Kit
Invitrogen™

SilverQuest™ Silver Staining Kit

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels.Read more
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Catalog number LC6070
Price (USD)
441.00
Each
Add to cart
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Price (USD)
441.00
Each
Add to cart
The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

Compare all silver stains ›

Features of this kit:
Compatible—staining compatible with mass spectrometry analysis
Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ∼30 minutes
Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeSilverQuest Silver Stain
Product TypeProtein Gel Staining Kit
Shelf Life6 Months
Product LineSilverQuest™
Quantity1 kit
Target MoleculeProtein
Unit SizeEach
Contents & Storage
This kit contains sufficient reagents to stain 25 minigels

Contains:
250 mL Sensitizer
25 mL Stainer
250 mL Developer
2 mL Developer Enhancer
250 mL Stopper solution
60 mL Destainer A
60 mL Destainer B

Store kit at room temperature (15°C to 30°C). Stable for 6 months.

Frequently asked questions (FAQs)

How much protein is required for mass spectrometry analysis by MALDI-TOF?

In general, the required amount needed to identify a gel-separated protein by enzymatic digestion (usually Trypsin) is about 2 pmole (equivalent to approximately 0.12 µg). Our SilverQuest Silver staining kit is mass spectrometry compatible.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and it took a longer time for band development resulting in a dark background. What happened?

Here are possible causes and solutions:

- Low protein load. Increase the amount of protein load. Be sure to have at least 1-5 ng protein on the gel.
- Some proteins may need longer fixing time. Increase the time for fixing the gel to 2 hours or overnight.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.