SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
Invitrogen™

SimplyBlue™ SafeStain

Green features
SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes.
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Catalog NumberDescription
LC6060SimplyBlue™ SafeStain, 1 L
LC6065SimplyBlue™ SafeStain, 3.5 L
Catalog number LC6060
Price (USD)
218.00
Each
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Description:
SimplyBlue™ SafeStain, 1 L
Request bulk or custom format
Price (USD)
218.00
Each
Add to cart
SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. SimplyBlue SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.
Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. SimplyBlue SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.

Features include:
• An easy-to-perform protocol that can be completed in less than three hours. For added speed, a simple microwave procedure can be completed in 12 minutes
• Destaining not required but may be performed with deionized water to achieve maximum sensitivity, especially when performing downstream analyses such as mass spectrometry, or when a crystal-clear background is required
• Sensitive—detect down to 7 ng of protein
• Fast—detect 1 μg of protein in as little as five minutes after staining
• Convenient—save valuable time by skipping reagent preparation with this ready-to-use formulation
• Non-hazardous—save money and hassle associated with hazardous shipping and disposal of hazardous waste

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionSimplyBlue™ SafeStain, 1 L
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeSafe Protein Stain
Shelf Life6 Months
Green FeaturesLess hazardous
Product LineSimplyBlue™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
SimplyBlue™ SafeStain is supplied as a 1X ready-to-use staining reagent. Store at room temperature. Guaranteed stable for 6 months when properly stored. There are no HazMat charges associated with this product.

Frequently asked questions (FAQs)

Can I use the Colloidal Blue Staining Kit (Cat. No. LC6025) for membrane staining to detect proteins that are transferred to a membrane?

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why is Coomassie G-250 used as the tracking dye in NuPAGE LDS Sample Buffer instead of bromophenol blue?

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the SimplyBlue SafeStain the appropriate stain for quantitation by densitometry?

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The sensitivity of my SimplyBlue SafeStain seems to be decreasing over time. Why is this?

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I destain proteins on a PVDF membrane that were stained with SimplyBlue SafeStain?

After staining with SimplyBlue SafeStain, use deionized water for the less strongly retained protein bands on the PVDF membrane.

Increasing methanol or ethanol concentrations up to 70% should destain any remaining bands. You can leave the membrane in the destain indefinitely.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SimplyBlue™ SafeStain FAQs