Colloidal Blue Staining Kit
Colloidal Blue Staining Kit
Invitrogen™

Colloidal Blue Staining Kit

The Colloidal Blue Staining Kit allows detection of nanogram levels of proteins in 1D or 2D PAGE gels with minimalRead more
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Catalog number LC6025
Price (USD)
311.00
Each
Add to cart
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Price (USD)
311.00
Each
Add to cart
The Colloidal Blue Staining Kit allows detection of nanogram levels of proteins in 1D or 2D PAGE gels with minimal effort and requires only water to destain. The Colloidal Blue stain uses colloidal chemistry that reduces free dye in solution and improves the protein-to-dye binding ratio. Samples are intensely stained and visible within three hours. Background staining is virtually eliminated by destaining overnight with water. The kit requires one easy solution preparation. Methanol is required in the staining step, but is not included in the kit.

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Staining Kit
Shelf Life6 Months
Quantity1 kit
Shipping ConditionRoom Temperature
Target MoleculeProtein
Unit SizeEach
Contents & Storage
The Colloidal Blue Staining Kit is supplied with sufficient stainer A and stainer B to stain 25 mini-gels. Store the kit at room temperature. The kit is guaranteed for 6 months when properly stored unless otherwise stated in the product documentation.

Frequently asked questions (FAQs)

Can I use the Colloidal Blue Staining Kit (Cat. No. LC6025) for membrane staining to detect proteins that are transferred to a membrane?

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why is Coomassie G-250 used as the tracking dye in NuPAGE LDS Sample Buffer instead of bromophenol blue?

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the SimplyBlue SafeStain the appropriate stain for quantitation by densitometry?

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The sensitivity of my SimplyBlue SafeStain seems to be decreasing over time. Why is this?

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I destain proteins on a PVDF membrane that were stained with SimplyBlue SafeStain?

After staining with SimplyBlue SafeStain, use deionized water for the less strongly retained protein bands on the PVDF membrane.

Increasing methanol or ethanol concentrations up to 70% should destain any remaining bands. You can leave the membrane in the destain indefinitely.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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