Novex™ IEF Cathode Buffer pH 3-7 (10X)
Novex™ IEF Cathode Buffer pH 3-7 (10X)
Invitrogen™

Novex™ IEF Cathode Buffer pH 3-7 (10X)

Novex™ IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex™ pH 3-7 IEFRead more
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Catalog number LC5370
Price (USD)
136.00
Each
Add to cart
Price (USD)
136.00
Each
Add to cart
Novex™ IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex™ pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferAnode & Cathode Buffers
Concentration10 X
Gel TypeIEF
pH Range3 to 7
Product TypeIEF Cathode Buffer
Gel CompatibilityNovex™ IEF Gels
Product LineNovex™
Quantity125 mL
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.