LIVE/DEAD™ Sperm Viability Kit
LIVE/DEAD™ Sperm Viability Kit
Invitrogen™

LIVE/DEAD™ Sperm Viability Kit

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm byRead more
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Catalog number L7011
Price (USD)
638.00
Each
Add to cart
Price (USD)
638.00
Each
Add to cart
The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR® 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant propidium iodide labels the nucleic acids of membrane-compromised sperm with red fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEukaryotic Cells, Sperm Cells
ColorGreen, Red
DescriptionLIVE/DEAD™ Sperm Viability Kit
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeSperm Viability Kit
Dye TypeOther Label(s) or Dye(s)
Emission516, 617 nm
Excitation Wavelength RangeVisible light
FormatTube(s), Slide(s)
Product LineLIVE/DEAD™
Quantity1 kit
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.