LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
Invitrogen™

LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. TargetRead more
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Catalog number L7010
Price (USD)
381.00
Each
Add to cart
Price (USD)
381.00
Each
Add to cart
The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells, Eukaryotic Cells
ColorGreen, Red
DescriptionLIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeCell-Mediated Cytotoxicity Kit
Dye TypeOther Label(s) or Dye(s)
Emission501, 617 nm
Excitation Wavelength Range484, 536 nm
FormatTube(s), Slide(s)
Product LineLIVE/DEAD™
Quantity1 kit
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in refrigerator at 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

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