LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
LIVE/DEAD&trade; <i>Funga</i>Light&trade; Yeast Viability Kit, for flow cytometry
Invitrogen™

LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutesRead more
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Catalog number L34952
Price (USD)
612.00
Each
Add to cart
Price (USD)
612.00
Each
Add to cart

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityImpermeant, Permeant
ColorGreen, Red
DescriptionLIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product TypeYeast Viability Kit
Dye TypeSYTO™ 9, propidium iodide
Emission498/617 nm
Excitation Wavelength Range485/535 nm
FormSolution
FormatTube(s)
Product LineFungaLight™, LIVE/DEAD™
Quantity200 Assays
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

What are the fluorescence excitation/emission maxima for the SYTO 9 dye and propidium iodide in the LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry?

SYTO 9 dye: 480/500 nm
Propidium iodide: 490/635 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.