FreeStyle™ 293 Expression System
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Invitrogen™

FreeStyle™ 293 Expression System

The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein.Read more
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Catalog number K900001
Price (USD)
1,768.00
Each
Add to cart
Price (USD)
1,768.00
Each
Add to cart
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO™ FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.

FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineFreeStyle™ 293-F Cells
ClassificationSerum-Free
Delivery TypeTransfection
Culture TypeSuspension Cell Culture
Cell Type293-derived Cells
Concentration1 x 10^7 cells/mL
Expression MechanismCell-Based Expression
Expression SystemMammalian
For Use With (Application)Protein Expression
Key FunctionsProtein Production
Product LineFreestyle™
Product TypeExpression System
Quantity1 Kit
Unit SizeEach
Contents & Storage
The FreeStyle™ 293 Expression System contains:
  • 1L GIBCO™ FreeStyle™ 293 Expression Medium
  • 1 x 107 FreeStyle™ 293-F Cells (frozen)
  • 1 mL 293fectin™ Transfection Reagent
  • 100 mL Opti-MEM™ I for Complex Formation
  • 25 μg pCMV-SPORT β-gal
Please review the FreeStyle™ 293 System Manual for storage and handling information. Store FreeStyle™ 293-F Cells in liquid nitrogen. Store 293fectin™ Transfection Reagents at 4°C. Store GIBCO™ FreeStyle™ 293 Expression Medium and Opti-MEM™ I at 2-8°C in the dark. Store pCMV SPORT β-gal at -20°C. Reagents are guaranteed stable for six months when stored properly.

Frequently asked questions (FAQs)

I am using your FreeStyle Max 293 Expression System and my cell viability is at most 87-90%. Is this normal? Do you have any tips to increase the cell viability? I am using 125 mL and 250 mL non-baffled, polycarbonate shaker flasks with vented caps. Will using baffled flasks help? Should I add Pluronic reagent to the medium?

Culture viability should be in the high 90% range for the seed stock that you are maintaining and growing for the transfections. After transfection, the viability will slowly decrease to the lower 90% to 80% range by about 3-6 days post-transfection and can drop more dramatically at 7 days or longer. This is when using our recommended protocol, which does not suggest any media changes or supplementation post-transfection.

A few parameters we recommend checking to assure high culture viability are: pH at ˜7.0, no visible clumping of the cells, CO2 levels at approximately 7-8% (this should be adjusted to achieve the appropriate pH), volume of culture at 40% or less of nominal flask capacity, and incubator temperature at 37 degrees C. If you have a shaker platform setup in your incubator, the platform may generate sufficient heat to increase the temperature of the incubator. We recommend using a thermometer attached to the shaker platform. Use of baffled flasks may help, if insufficient oxygenation of the cultures is suspected. We also recommend handling and storing the culture media properly, as light exposure can result in breakdown of certain media components. If you are using FreeStyle 293 Expression Medium, supplementing with Pluronic reagent is not necessary.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the main difference between the FreeStyle Max 293 Expression System and the FreeStyle 293 Expression System?

The two systems have the same components except for the transfection reagent and the medium supplied for formation of transfection complexes. The FreeStyle Max 293 Expression System contains FreeStyle Max Reagent and OptiPRO SFM (serum-free medium that is devoid of animal-origin components), whereas the FreeStyle 293 Expression System contains 293fectin Transfection Reagent and Opti-MEM I Reduced Serum Medium (serum-free medium but not animal-origin free).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Which vectors do you recommend for use with FreeStyle system?

Generally, any mammalian expression vector should be compatible with the FreeStyle expression system. Some of the more popular choices include pcDNA3.3-TOPO TA vector (Cat. No. K8300-01), pcDNA3.4-TOPO TA vector (Cat. no. A14697), pcDNA3.2 DEST vector (Cat. No. 12489018), pCEP vector (Cat. No. V044-50), or pcDNA4/HisMax vector (Cat. No. K864-20).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between FreeStyle 293 Expression Medium and FreeStyle F17 Expression Medium?

FreeStyle F17 Expression Medium was designed from a different base formulation than FreeStyle 293 Expression Medium. FreeStyle F17 medium shows equal or better performance than the FreeStye 293 medium for certain cell lines and proteins. It is capable of supporting the growth of 293 and CHO cells and offers a wider variety and selection of transient cell culture media options.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.