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ViraPower™ Lentiviral Packaging Mix
ViraPower™ Lentiviral Packaging Mix contains an optimized mixture of the three packaging plasmids, pLP1, pLP2, and pLP/VSVG. These plasmids supplyRead more
Catalog number K497500
Price (USD)
3,565.00
Each
Price (USD)
3,565.00
Each
ViraPower™ Lentiviral Packaging Mix contains an optimized mixture of the three packaging plasmids, pLP1, pLP2, and pLP/VSVG. These plasmids supply the helper functions as well as structural and replication proteins in trans required to produce a recombinant lentivirus, containing your gene of interest, using our ViraPower™ Lentiviral Expression System.
The ViraPower™ Lentiviral Expression System
The ViraPower™ Lentiviral Expression System allows creation of a replication-incompetent, HIV-1–based lentivirus that is used to deliver and express your gene of interest in either dividing or non-dividing mammalian cells. The major components of the system include:
• An expression plasmid containing the gene of interest under the control of a choice of promoters, and elements that allow packaging of the construct into virions
• An optimized mix of the three packaging plasmids (pLP1, pLP2, and pLP/VSVG) that supply the structural and replication proteins in trans that are required to produce the lentivirus
• The 293FT cell line, which allows production of lentivirus following cotransfection of the expression plasmid and the plasmids in the packaging mix
• Control expression plasmid to optimize virus production and cell transduction, containing either the lacZ gene—which when packaged into virions and transduced into a mammalian cell line, expresses β-galactosidase (included with each expression vector), or the Emerald Green Fluorescent Protein (EmGFP) gene—which when packaged into virions and transduced into a mammalian cell line, expresses EmGFP.
The ViraPower™ Lentiviral Expression System
The ViraPower™ Lentiviral Expression System allows creation of a replication-incompetent, HIV-1–based lentivirus that is used to deliver and express your gene of interest in either dividing or non-dividing mammalian cells. The major components of the system include:
• An expression plasmid containing the gene of interest under the control of a choice of promoters, and elements that allow packaging of the construct into virions
• An optimized mix of the three packaging plasmids (pLP1, pLP2, and pLP/VSVG) that supply the structural and replication proteins in trans that are required to produce the lentivirus
• The 293FT cell line, which allows production of lentivirus following cotransfection of the expression plasmid and the plasmids in the packaging mix
• Control expression plasmid to optimize virus production and cell transduction, containing either the lacZ gene—which when packaged into virions and transduced into a mammalian cell line, expresses β-galactosidase (included with each expression vector), or the Emerald Green Fluorescent Protein (EmGFP) gene—which when packaged into virions and transduced into a mammalian cell line, expresses EmGFP.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
PromoterRSV, CMV
Product TypeLentiviral Packaging Mix
Selection Agent (Eukaryotic)Blasticidin
Protein TagV5 Epitope Tag
Cloning MethodTOPO-TA, Gateway
Quantity60 reactions
VectorpLP
Product LineViraPower™
For Use With (Application)Viral Expression
Unit SizeEach
Contents & Storage
Three tubes, each containing 195 μg lyophilized mix. The packaging mix is enough for 60 transfection reactions in 10 cm tissue culture dishes. Store at -20°C.