PureLink™ PCR Purification Kit
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PureLink™ PCR Purification Kit
PureLink™ PCR Purification Kit
PureLink™ PCR Purification Kit
Invitrogen™

PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. AfterRead more
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Catalog NumberQuantity
K31000150 Preps
K310002250 Preps
Catalog number K310001
Price (USD)
133.00
Each
Add to cart
Quantity:
50 Preps
Price (USD)
133.00
Each
Add to cart

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:
• 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
• No need for tedious sample pH adjustments necessary with other kits
• Convenient and rapid protocol, completed in less than 10 minutes
• Optional size-selection buffers included for selectively purifying 300–600 bp fragments or removing fragments <300 bp

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
High-throughput CompatibilityHigh-throughput Compatible
Product LinePureLink™
Product TypePCR Purification Kit
Quantity50 Preps
Run Time10 min.
Shipping ConditionRoom Temperature
TargetDNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)Next-Generation Sequencing (NGS), Cloning
Isolation TechnologySilica Spin Column
Sample TypePCR Products
Unit SizeEach
Contents & Storage
• 15 mL Binding Buffer (B2)
• 23 mL Binding Buffer High-Cutoff (B3)
• 16 mL Wash Buffer (W1)
• 15 mL Elution Buffer (E1)
• 50 PureLink™ PCR Spin Columns with Collection Tubes
• 50 PureLink™ Elution Tubes (1.7 mL)

Store at room temperature.

Frequently asked questions (FAQs)

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.

What is nested and semi-nested PCR?

Nested PCR requires two separate amplifications, with the first one using one set of PCR primers and the second one using internal, "nested" primers and 1% or less of the first PCR reaction as a template. Nested PCR is used when the target is present in low abundance or when non-specific PCR products are being produced along with the specific product. Semi-nested PCR is used when there is only enough sequence information to make a primer internal to one end of the primary PCR product such as in RACE (rapid amplification of cDNA ends).

Can I sequence PCR products rather than plasmids, and what is the advantage?

Yes, you can sequence PCR products. This is an excellent way to confirm the sequence of an insert when plasmid sequencing produces an unexpected sequence. The target DNA is amplified with a single set of primers and then sequenced using the same primers (although not all PCR primers work well as sequencing primers). Plasmid DNA can be PCR amplified using a one set of primers and the resulting product can then be purified using a column and then sequenced using the same primers. You can even get more specificity by using a sequencing primer that binds internally to one of the PCR primers.

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