Luminaris HiGreen qPCR Master Mix
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Thermo Scientific™

Luminaris HiGreen qPCR Master Mix

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberSufficient For
K0991250 Reactions
K0992500 Reactions
K0993250 Reactions
K09945000 Reactions
Catalog number K0991
Price (USD)
290.00
Each
Add to cart
Sufficient For:
250 Reactions
Price (USD)
290.00
Each
Add to cart
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights:

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
—Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
—Sensitivity: single copy detection
—Wide linear range: accurate quantification across 9 orders of magnitude
—Reproducibility and convenience: ready-to-use 2X master mix

Applications:

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes:

Luminaris HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodSYBR
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product LineLuminaris
Product TypeqPCR Master Mix
Sufficient For250 Reactions
For Use With (Application)Gene Expression
Concentration2X
Fidelity (vs. Taq)2 X
No. of Reactions250 Reactions
Sample TypeDNA, dsDNA
Unit SizeEach
Contents & Storage
Contains:
• 2 x 1.25 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 250 x 20 μL reactions
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

Frequently asked questions (FAQs)

Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?

The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.

What DNA-binding dye is present in Luminaris Color HiGreen qPCR Master Mix?

Luminaris Color HiGreen master mixes contain SYBR Green I double-stranded DNA binding dye.

Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?

Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.

What enzyme is used in Luminaris qPCR Master Mixes?

Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.

Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?

When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.