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Thermo Scientific™
GeneJET Stabilized and Fresh Whole Blood RNA Kit
Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high qualityRead more
Catalog number K0871
Price (USD)
355.00
Each
Price (USD)
355.00
Each
Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
Highlights
• Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
• Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient—up to 4 μg of total RNA from 500 μL of healthy human blood
• Pure—isolates intact total RNA free of any RT-qPCR inhibitors
Applications
Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting
Includes
• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL
Related Products
Lysis Buffer for GeneJET Blood RNA Kit
Wash Buffer 1 for GeneJET Blood RNA Kit (concentrated)
Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
Highlights
• Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
• Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient—up to 4 μg of total RNA from 500 μL of healthy human blood
• Pure—isolates intact total RNA free of any RT-qPCR inhibitors
Applications
Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting
Includes
• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL
Related Products
Lysis Buffer for GeneJET Blood RNA Kit
Wash Buffer 1 for GeneJET Blood RNA Kit (concentrated)
Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
FormatSpin Column
Isolation TechnologySpin Column
Purification Time30 min.
Sample TypeBlood
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
No. of Reactions50 Preps
Product LineGeneJET
TargetTotal RNA
Unit SizeEach