iBlot™ 3 Transfer Stacks, midi, PVDF
iBlot™ 3 Transfer Stacks, midi, PVDF
iBlot™ 3 Transfer Stacks, midi, PVDF
iBlot™ 3 Transfer Stacks, midi, PVDF
iBlot™ 3 Transfer Stacks, midi, PVDF
iBlot™ 3 Transfer Stacks, midi, PVDF
Invitrogen™

iBlot™ 3 Transfer Stacks, midi, PVDF

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iBlot 3 Transfer Stacks are used with the iBlot 3 Western Blot Transfer Device for the transfer of proteins fromRead more
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Catalog NumberQuantity
IB3400110 Stacks
IB34001X33 x 10 Stacks
Catalog number IB34001
Price (USD)
290.00
Each
Add to cart
Quantity:
10 Stacks
Price (USD)
290.00
Each
Add to cart

iBlot 3 Transfer Stacks are used with the iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments. iBlot 3 Transfer Stacks contain copper-fiber electrode sheets, optimized cathode and anode solid buffer gels, and a preconditioned PVDF transfer membrane. They are compatible with common commercially available protein gels, including Bis-Tris, Tris-Glycine, Tris-Acetate, and Tricine chemistries. This midi-size stack (13 x 8.3 cm) can be used with midi-size gels or two mini-size gels.

The iBlot 3 Western Blot Transfer System (iBlot 3 Transfer Stacks plus iBlot3 Transfer Device) has been optimized to provide protein transfers in 3–8 minutes that produce higher quality and more reliable western blot result compared to many other transfer methods.

PVDF membrane

The PVDF membrane (0.2-μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. Proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 μg/cm2.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Length (Metric)13.5 cm
Width (Metric)8 cm
Quantity10 Stacks
FormatTransfer Stack
MaterialPVDF
Pore Size0.2 μm
Dimensions (LxW)13.5 x 8 cm (Midi)
Product LineiBlot
Sufficient For10 Blots
Green FeaturesLess waste, sustainable packaging, sustainable disposal
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

I used PVDF Gel Transfer Stacks with the iBlot 3 Western Blot Transfer System, and my proteins did not transfer to the membrane. What happened?

This is likely due to the PVDF membrane being dry or partially dry. Regions where PVDF membranes are dry appear whiter than places where the membrane is wet. Remove the membrane and reactivate in 100% methanol, and rinse in water before reapplying to the transfer stack.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

After transferring using the iBlot 3 Western Blot Transfer System, I noticed that the Bottom Stack transfer gel melted to a viscous blue solution. Why is this?

This happens if the membrane is trimmed to fit the gel size resulting in direct contact between the Top and Bottom transfer stacks. This can be avoided by maintaining the membrane size identical to the transfer stack. Transfer quality is not affected by smaller gel size compared to the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the Blotting Roller that is provided with the iBlot 3 Western Blot Transfer System?

The Blotting Roller is a plastic roller attached to a stainless steel handle. It is used to remove any air bubbles between the gel and blotting membrane during the assembly of the transfer stacks and gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the membrane in the iBlot 3 Transfer Stacks to fit my separating gel?

No, we recommend maintaining the membrane size identical to the transfer stacks. This helps ensure that there is no direct contact between the Top and Bottom transfer stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are the PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks compatible with fluorescent antibodies or membrane staining (e.g., LI-COR, Ponceau S)?

The PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks are compatible with all commonly used detection methods such as staining, immunodetection, fluorescence, etc.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ 3 Transfer Stacks, midi, PVDF FAQs