iBlot™ 2 Transfer Stacks, nitrocellulose, mini

Like the iBlot 2 Dry Blotting System? Then you'll love our iBlot 3 Dry Blotting System which enables twice the throughput and features optional built-in cooling for better consistency. Learn more about the benefits of the iBlot 3 here.

Invitrogen™

iBlot™ 2 Transfer Stacks, nitrocellulose, mini

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacksRead more

Catalog Number	Quantity
IB23002	10 Stacks

Catalog number IB23002

Price (USD)

226.00

Add to cart

Quantity:

10 Stacks

Price (USD)

226.00

Add to cart

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 x 8 cm) can be used with one mini-size gel.

Nitrocellulose membrane

The nitrocellulose membrane (0.2-μm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 μg/cm².

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Length (Metric)8 cm

Width (Metric)8 cm

Quantity10 Stacks

FormatTransfer Stack

MaterialNitrocellulose

Pore Size0.2 μm

Dimensions (LxW)8 x 8 cm

Product LineiBlot

Sufficient For10 Blots

Green FeaturesLess waste, responsibly packaged, extended life

Unit SizeEach

Contents & Storage

Store at room temperature.

Frequently asked questions (FAQs)

If a green color appears on the membrane after the transfer using the iBlot 2 Dry Blotting System, will it affect the results?

The green discoloration is copper deposits from the transfer stack, and it does not affect the results. To minimize this effect, shake excess water off the filter paper and buffer from the gel before placing each on the stack. The current formulation of stacks minimizes the green discoloration.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the bottom stack of my iBlot 2 Transfer Stack to fit my separating gel?

No. Do not trim the iBlot 2 Transfer Stacks to fit your gel size.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the PVDF membrane in the iBlot 2 PVDF Gel Transfer Stack pre-activated?

The PVDF membrane is pre-activated and ready for use without any pre-treatment with alcohol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the stack and/or absorbent pad more than once when using the iBlot 2 Dry Blotting System?

No. The transfer stacks have a finite amount of ions to drive the transfer and are depleted after a single use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one when using the iBlot 2 Dry Blotting System?

Yes. The device is designed to do so with no impact on performance.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ 2 Transfer Stacks, nitrocellulose, mini FAQs