Indo-1, AM, cell permeant - Special Packaging
Indo-1, AM, cell permeant - Special Packaging
Invitrogen™

Indo-1, AM, cell permeant - Special Packaging

This cell-permeant indo-1, AM is a UV light—excitable, ratiometric Ca2+ indicator. The emission maximum of indo-1 shifts from ∼475 nmRead more
Have Questions?
Catalog number I1223
Price (USD)
331.00
Each
Add to cart
Price (USD)
331.00
Each
Add to cart
This cell-permeant indo-1, AM is a UV light—excitable, ratiometric Ca2+ indicator. The emission maximum of indo-1 shifts from ∼475 nm in Ca2+-free medium to ∼400 nm when the dye is saturated with Ca2+. This AM ester form of the dye is available in 1mg amounts (I-1203) and in 1 mM solution (I-1226). A cell-impermeant pentapotassium (F-1202) salt form of the dye is also available.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Cell Viability, Proliferation and Cellular Imaging, Flow Cytometry
For Use With (Equipment)Fluorescence Microscope, Microphotometer, Flow Cytometer
Product TypeIndo-1 AM
Dye TypeFluorescent Dye-Based
FormSolid
Quantity20 x 50 μg
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What indicator dye can I use to measure calcium flux by flow cytometry?

Indo-1, AM is the preferred stain for flow cytometry, where it is more practical to use a single laser for excitation, usually the 351–364 nm spectral lines of the argon-ion laser, and monitor two emissions. The emission maximum of Indo-1 shifts from ~475 nm in Ca2+-free medium to ~400 nm when the dye is saturated with Ca2+. Indo-1, AM is particularly suited for multicolor fluorescence applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.