Image-IT™ DEAD Green™ Viability Stain
Image-IT™ DEAD Green™ Viability Stain
Invitrogen™

Image-IT™ DEAD Green™ Viability Stain

Image-iT DEAD Green viability stain is a fast, sensitive and easy-to-use viability indicator that is also amenable to fixation andRead more
Have Questions?
Catalog number I10291
Price (USD)
457.00
Each
Add to cart
Price (USD)
457.00
Each
Add to cart
Image-iT DEAD Green viability stain is a fast, sensitive and easy-to-use viability indicator that is also amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of cytotoxicity. Image-iT DEAD Green is an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Concentration1 mM
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope
Product TypeViability Stain
EmissionVisible
FormLiquid
Product LineImage-iT™, Molecular Probes™
Quantity1 Vial
Shipping ConditionWet Ice
Sub Cellular LocalizationNucleus
Unit SizeEach
Contents & Storage
The stain includes sufficient material to perform 10 plates in a 96-well plate format and 50 μL well volume.
  • Store at ≤ −20°C, desiccate and protect from light.

    • Frequently asked questions (FAQs)

    Do you offer a cell viability stain compatible with fixation?

    Our Image-IT DEAD Green Viability Stain (Cat. No. I10291) is amenable to fixation and permeabilization after the application of the stain. It is important to use fixatives free of alcohol or other organic solvents.

    Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

    Which cell viability kits are compatible with fixation?

    The LIVE/DEAD Fixable kits for flow cytometry analysis are compatible with fixation. These kits use amine-reactive cell-impermeant dyes that stain the cell surface of live cells and also the cytosol of dead cells-live cells are dim and dead cells are bright. Since the dye is covalently bound to the cells, it will be retained after fixation. Unfortunately, this method does not work well for imaging-based assays, as all cells are stained and it is difficult to distinguish bright dead cells from dim live cells with a microscope. Ethidium monoazide (EMA; Cat No. E1374) is a cell impermeant nucleic acid stain that can be applied to live cultures and stains only dead cells. After incubation and washing away unbound dye, the cells can be exposed to light to photoactivate EMA to crosslink to dead cell DNA. After crosslinking to dead cell DNA, the samples may be fixed and permeabilized. Image-IT DEAD Green Viability Stain (Cat. No. I10291) for imaging and high-content screening (HCS) analysis is a live-cell impermeant DNA binding dye that is compatible with fixation and permeabilization with good retention up to 48 hours. We also have a LIVE/DEAD Reduced Biohazard Cell Viability Kit (Cat. No. L7013) for imaging and flow analysis that contains two DNA binding dyes, SYTO 10 and Dead Red, that are sufficiently retained to be analyzed soon after 4% glutaraldehyde fixation.
    Note: In general, DNA-binding dyes and calcein AM are not compatible with fixation, as these dyes are not covalently bound to components of the cell and will thus slowly diffuse out of cells after fixation, gradually staining all cells as dead.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.