HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging
HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging
Invitrogen™

HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/orRead more
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Catalog number H34477
Price (USD)
534.00
Each
Add to cart
Price (USD)
534.00
Each
Add to cart
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorDeep Red
For Use With (Equipment)High Content Instrument
Quantity1 each
Detection MethodFluorescence
Product LineLipidTOX™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeLipid Stain
SubCellular LocalizationCell Membranes, Lipids
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the HCS LipidTOX Deep Red Neutral Lipid Stain (Cat. No. H34477) ready to use, or do I need to dilute it in 1X PBS or DMSO?

The HCS LipidTOX Deep Red Neutral Lipid Stain is provided as a solution in DMSO and needs to be diluted before use. The recommended dilution is 1:200 in 1X PBS or other buffer (e.g. HBSS, TBS, etc).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffer should I use with HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging (Cat. No. H3447)?

The ideal buffer with HCS LipidTOX Deep Red Neutral Lipid Stain is PBS at pH 7.4.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.