FUN™ 1 Cell Stain
FUN™ 1 Cell Stain
Invitrogen™

FUN™ 1 Cell Stain

FUN™ 1 is a unique two-color fluorescent viability probe for yeast and fungi. The FUN™ 1 stain passively diffuses intoRead more
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Catalog number F7030
Price (USD)
346.00
Each
Add to cart
Price (USD)
346.00
Each
Add to cart
FUN™ 1 is a unique two-color fluorescent viability probe for yeast and fungi. The FUN™ 1 stain passively diffuses into a variety of cell types and initially stains the cytoplasm with a diffusely distributed green fluorescence. However, in several common species of yeast and fungi, subsequent processing of the dye by live cells results in the formation of distinct vacuolar structures with compact form that exhibit a striking red fluorescence, accompanied by a reduction in the green cytoplasmic fluorescence. Formation of the intravacuolar structures requires both plasma membrane integrity and metabolic capability. Dead cells fluoresce bright yellow-green, with no discernable red structures.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeFungal Cells, Yeast Cells
Concentration10 mM
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeStain
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Product LineFUN™
Quantity100 μL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Can FUN 1 stained cells be examined by flow cytometry?

Yes. Use a 488 nm laser line and standard FITC and PE channels for two-color detection of green (dead/metabolically inactive cells) and red (live, metabolically active cells) emission.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?

No. FUN 1 accumulates into vacuoles by an unknown transport pathway, but any mutants/ recombinant cells or experimental treatments that result in a deficiency or block in vesicle-mediate transport into vacuoles may result in cells that do not have red vacuoles, even though the cells are live and metabolically active. For more information see J Microbiol Methods 78:208 (2009).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.