Phusion Blood Direct PCR Kit
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Phusion Blood Direct PCR Kit
Thermo Scientific™

Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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Catalog NumberNo. of Reactions
F547S100 Reactions
F547L500 Reactions
Catalog number F547S
Price (USD)
154.00
Each
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No. of Reactions:
100 Reactions
Request bulk or custom format
Price (USD)
154.00
Each
Add to cart
Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.

This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Simple protocol requires minimal hands-on time
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
PolymerasePhusion Blood II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Concentration2 U/μL
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypeBlood
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Phusion Blood II DNA Polymerase, 40 μL
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

What is the highest blood concentration possible in the reaction when using Phusion Blood Direct PCR Master Mix and Kit?

We recommend using blood concentration up to 20%; however, we had successful amplifications with up to 40% of blood in the reaction mix. In some cases optimization may be required to achieve this.