Fura Red™, AM, cell permeant
Fura Red™, AM, cell permeant
Invitrogen™

Fura Red™, AM, cell permeant

Fura Red, AM is a visible light—excitable fura-2 analog that offers unique possibilities for ratiometric measurement of Ca2+ in singleRead more
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Catalog NumberQuantity
F302110 x 50μg
F3020500μg
Catalog number F3021
Price (USD)
664.00
Each
Add to cart
Quantity:
10 x 50μg
Price (USD)
664.00
Each
Add to cart
Fura Red, AM is a visible light—excitable fura-2 analog that offers unique possibilities for ratiometric measurement of Ca2+ in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in 1 mg amounts (F-3020).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Cell Viability and Proliferation
For Use With (Equipment)Fluorescence Microscope, Microphotometer, Flow Cytometer
Product TypeFura Red AM
Dye TypeFluorescent Dye-Based
Product LineFura Red™
Quantity10 x 50μg
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

When using Calcium Crimson, AM, as an indicator of intracellular calcium flux, I am not getting a good degree of change. The cells also have GFP. What can I do?

This is a known drawback of Calcium Crimson, AM (as well as Calcium Orange, AM and Fura Red, AM, which are also in the same emission range). You can try increasing the concentration and washing out of any unlabeled dye from the media, to try to get better signal-to-background. If that fails, we recommend using Rhod-3 AM instead, which has a much better change in signal in that wavelength.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find the manual for the Fura Red, AM, cell permeant (Cat. No. F3020, F3021)?

Unfortunately, we do not have a user manual for the Fura Red, AM, cell permeant (Cat. No. F3021). You can reference section 19.3 of the Molecular Probes handbook for recommendations for use.
There is also a large number of citations & references provided on the product page.

Basically, you can dissolve the product in good quality anhydrous DMSO to prepare a stock solution of 1-10 mM and use a final solution of about 1-10 µM in a physiological buffer. You can then incubate for 15 to 60 minutes, wash and then leave the cells for about 30 minutes to allow the AM group to hydrolyse before starting the assay. You can add Pluronic F-127 (e.g., Cat. No. P6866) to facilitate uptake. Store the DMSO stock solution at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.