Phire Plant Direct PCR Kit (without sampling tools)
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Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific™

Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
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Catalog number F130WH
Price (USD)
369.00
Each
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Price (USD)
369.00
Each
Add to cart
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria.

The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions. The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Very little sample material required
  • Two simple protocols for various applications
  • Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb)

Applications

  • Genotyping
  • Transgene detection

Notes

  • The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
PolymerasePhire Hot Start II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypePlant
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Phire Hot Start II DNA Polymerase, 200 μL
• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL
• Universal Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

Do the Direct PCR Master Mixes and Kits come with tissue punchers?

No, tissue punchers are not provided. You can purchase 0.3 mm Tissue Punchers (Cat. No. F-200S), or cut the sample with a scalpel. Some scientists use 100 µL pipette tips to take a sample.

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